Multifaceted RNA sequencing
RNA has long been an obvious target for understanding the inner workings of a cell. The advances in sequencing technologies from the recent decade have opened the door to this once elusive messenger of the cellular command chain. RNA sequencing can reveal many aspects of cellular mechanism, such as alternative splicing, post-transcriptional modifications, mutations and SNPs, changes in gene expressions, and even profiling cell populations based on the outcome. It is also a versatile method that can help delving into the RNA biology by looking at different types of RNAs, and their modifications. Aided by the development of single cell technologies, RNA-seq is becoming an ever so powerful tool to unravel the individuality of living cells and organisms.
We offer a variety of RNA-seq solutions. As with all our services, we promise easy sample submission, fast turnaround time, and expert customer services.
Long-reads options:
Direct RNA sequencing by Nanopore
Direct RNA sequencing workflow at-a-glance
The recently-updated Direct RNA sequencing kit by Nanopore offers a unique opportunity to sequence full-length RNA molecules as is, without any PCR amplification.
The reaction is done on Nanopore’s PromethION sequencer, and can provide up to 40 million reads in one run.
The sample prep requires a cDNA synthesis step, but the resulting cDNA strand is used to stabilize the complex instead of being sequenced.
Image source: nanoporetech.com
cDNA-PCR sequencing by Nanopore
cDNA-PCR sequencing workflow at-a-glance
The cDNA-PCR RNA sequencing kit allows RNA input as low as 10ng, and multiplexing up to 24 samples in one flow cell.
The workflow can also be adapted to include custom barcodes and UMI’s, Reverse-transcribed RNA or other RNA species lacking poly-A tail can also be used as input after additional poly-adenylation.
Image source: nanoporetech.com
Iso-seq by Pacbio
Iso-seq workflow at-a-glance
With Single Molecule, Real-Time (SMRT®) sequencing on Sequel IIe system, you can complete transcript isoforms in genes of interest or across the entire transcriptome. The Iso-Seq® method allows users to generate full-length cDNA sequences up to 10 kb in length — with no assembly required — to confidently characterize full-length transcript isoforms.
Image source: pacb.com
Kinnex -seq by Pacbio
Kinnex-seq workflow at-a-glance
The Kinnex kits are based on the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries to achieve higher throughput. Depending on applications, 8 to 12 amplicons can be joined into Kinnex array, to obtain 15 to 40 million reads per run.
Three types of Kinnex kits are available for full-length RNA, single-cell RNA, and 16S rRNA sequencing on PacBio long-read sequencing systems, which enable large-scale studies at a resolution difficult to attain with short-read RNA sequencing.
Full length RNA sequencing
Single-cell RNA sequencing
16S rRNA sequencing
Image source: pacb.com
Short-reads options:
Bulk RNA-seq by Illumina
We accept samples in various formats: total RNA, FFPE, blood, fresh/frozen tissue, cell pellet, etc.
Customizable coverage on Illumina platforms.
In-depth discussion prior to any project
On-demand bioinformatic analysis
Single-cell (sc) RNA-seq
10X Genomics platform available for single cell processing
SMART-Seq® technologies
Customizable coverage on Illumina platforms.
In-depth discussion prior to any project
On-demand bioinformatic analysis
